Association of COPD exacerbation frequency with gene expression of pattern recognition receptors in inflammatory cells in induced sputum
Identifieur interne : 001031 ( Main/Exploration ); précédent : 001030; suivant : 001032Association of COPD exacerbation frequency with gene expression of pattern recognition receptors in inflammatory cells in induced sputum
Auteurs : Daisuke Kinose [Japon] ; Emiko Ogawa [Japon] ; Megumi Kudo [Japon] ; Satoshi Marumo [Japon] ; Hirofumi Kiyokawa [Japon] ; Yuma Hoshino [Japon] ; Toyohiro Hirai [Japon] ; Kazuo Chin [Japon] ; Shigeo Muro [Japon] ; Michiaki Mishima [Japon]Source :
- The Clinical Respiratory Journal [ 1752-6981 ] ; 2016-01.
Abstract
Background: Bacteria and viruses are major causes of chronic obstructive pulmonary disease (COPD) exacerbations. Molecular components of these pathogens are recognized by pattern‐recognition receptors (PRRs) expressed by various cells in the airway, which leads to initiation of inflammatory processes. Expression levels of PRRs in airway inflammatory cells are expected to affect susceptibility to COPD exacerbation. Aims: This prospective observational study was conducted to detect any association between exacerbation and PRR expression. Methods: Thirty‐one male COPD patients were recruited. At baseline, clinical history, lung function measurements, peripheral blood samples and induced sputum were obtained. Using sputum samples, we performed gene expression analysis of TLR2, TLR3, TLR4, NOD1, NOD2, RIG‐I and MDA‐5 by quantitative reverse transcription‐polymerase chain reaction in addition to quantitative bacterial culture. COPD exacerbations were assessed based on Anthonisen's criteria using symptom diaries for the following 1‐year period. Results: During 1‐year follow‐up period, 13 patients experienced at least one exacerbation, but 18 patients did not. Those with exacerbations tended to be more severe COPD and showed larger neutrophil fraction in their induced sputum. Among PRRs, only TLR3 gene expression was increased in COPD patients with exacerbation compared with those without exacerbations. Multivariate logistic regression analysis including neutrophil fraction and TLR3 gene expression as predictor variables demonstrated that only an increase of neutrophil fraction, but not TLR3 gene expression, was a significant predictor for COPD exacerbation. Conclusion: TLR3 expression in inflammatory cells might affect the susceptibility to COPD exacerbation.
Url:
DOI: 10.1111/crj.12171
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000774
- to stream Istex, to step Curation: 000741
- to stream Istex, to step Checkpoint: 000012
- to stream Main, to step Merge: 001033
- to stream Main, to step Curation: 001031
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Association of COPD exacerbation frequency with gene expression of pattern recognition receptors in inflammatory cells in induced sputum</title><author><name sortKey="Kinose, Daisuke" sort="Kinose, Daisuke" uniqKey="Kinose D" first="Daisuke" last="Kinose">Daisuke Kinose</name></author><author><name sortKey="Ogawa, Emiko" sort="Ogawa, Emiko" uniqKey="Ogawa E" first="Emiko" last="Ogawa">Emiko Ogawa</name></author><author><name sortKey="Kudo, Megumi" sort="Kudo, Megumi" uniqKey="Kudo M" first="Megumi" last="Kudo">Megumi Kudo</name></author><author><name sortKey="Marumo, Satoshi" sort="Marumo, Satoshi" uniqKey="Marumo S" first="Satoshi" last="Marumo">Satoshi Marumo</name></author><author><name sortKey="Kiyokawa, Hirofumi" sort="Kiyokawa, Hirofumi" uniqKey="Kiyokawa H" first="Hirofumi" last="Kiyokawa">Hirofumi Kiyokawa</name></author><author><name sortKey="Hoshino, Yuma" sort="Hoshino, Yuma" uniqKey="Hoshino Y" first="Yuma" last="Hoshino">Yuma Hoshino</name></author><author><name sortKey="Hirai, Toyohiro" sort="Hirai, Toyohiro" uniqKey="Hirai T" first="Toyohiro" last="Hirai">Toyohiro Hirai</name></author><author><name sortKey="Chin, Kazuo" sort="Chin, Kazuo" uniqKey="Chin K" first="Kazuo" last="Chin">Kazuo Chin</name></author><author><name sortKey="Muro, Shigeo" sort="Muro, Shigeo" uniqKey="Muro S" first="Shigeo" last="Muro">Shigeo Muro</name></author><author><name sortKey="Mishima, Michiaki" sort="Mishima, Michiaki" uniqKey="Mishima M" first="Michiaki" last="Mishima">Michiaki Mishima</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:94F27A970F6FC3B47ED89F596AFC6157509AD2DA</idno><date when="2016" year="2016">2016</date><idno type="doi">10.1111/crj.12171</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-V7Z7HK2J-2/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000774</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000774</idno><idno type="wicri:Area/Istex/Curation">000741</idno><idno type="wicri:Area/Istex/Checkpoint">000012</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000012</idno><idno type="wicri:doubleKey">1752-6981:2016:Kinose D:association:of:copd</idno><idno type="wicri:Area/Main/Merge">001033</idno><idno type="wicri:Area/Main/Curation">001031</idno><idno type="wicri:Area/Main/Exploration">001031</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Association of COPD exacerbation frequency with gene expression of pattern recognition receptors in inflammatory cells in induced sputum</title><author><name sortKey="Kinose, Daisuke" sort="Kinose, Daisuke" uniqKey="Kinose D" first="Daisuke" last="Kinose">Daisuke Kinose</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Ogawa, Emiko" sort="Ogawa, Emiko" uniqKey="Ogawa E" first="Emiko" last="Ogawa">Emiko Ogawa</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Health Administration Center and Division of Respiratory Medicine, Department of Medicine, Shiga University of Medical Science, Otsu</wicri:regionArea><wicri:noRegion>Otsu</wicri:noRegion></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Japon</country></affiliation></author><author><name sortKey="Kudo, Megumi" sort="Kudo, Megumi" uniqKey="Kudo M" first="Megumi" last="Kudo">Megumi Kudo</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Marumo, Satoshi" sort="Marumo, Satoshi" uniqKey="Marumo S" first="Satoshi" last="Marumo">Satoshi Marumo</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Kiyokawa, Hirofumi" sort="Kiyokawa, Hirofumi" uniqKey="Kiyokawa H" first="Hirofumi" last="Kiyokawa">Hirofumi Kiyokawa</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Hoshino, Yuma" sort="Hoshino, Yuma" uniqKey="Hoshino Y" first="Yuma" last="Hoshino">Yuma Hoshino</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Hirai, Toyohiro" sort="Hirai, Toyohiro" uniqKey="Hirai T" first="Toyohiro" last="Hirai">Toyohiro Hirai</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Chin, Kazuo" sort="Chin, Kazuo" uniqKey="Chin K" first="Kazuo" last="Chin">Kazuo Chin</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Care and Sleep Control Medicine, Graduate School of Medicine, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Muro, Shigeo" sort="Muro, Shigeo" uniqKey="Muro S" first="Shigeo" last="Muro">Shigeo Muro</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Mishima, Michiaki" sort="Mishima, Michiaki" uniqKey="Mishima M" first="Michiaki" last="Mishima">Michiaki Mishima</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The Clinical Respiratory Journal</title><title level="j" type="alt">THE CLINICAL RESPIRATORY JOURNAL</title><idno type="ISSN">1752-6981</idno><idno type="eISSN">1752-699X</idno><imprint><biblScope unit="vol">10</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="11">11</biblScope><biblScope unit="page" to="21">21</biblScope><biblScope unit="page-count">11</biblScope><date type="published" when="2016-01">2016-01</date></imprint><idno type="ISSN">1752-6981</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1752-6981</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Background: Bacteria and viruses are major causes of chronic obstructive pulmonary disease (COPD) exacerbations. Molecular components of these pathogens are recognized by pattern‐recognition receptors (PRRs) expressed by various cells in the airway, which leads to initiation of inflammatory processes. Expression levels of PRRs in airway inflammatory cells are expected to affect susceptibility to COPD exacerbation. Aims: This prospective observational study was conducted to detect any association between exacerbation and PRR expression. Methods: Thirty‐one male COPD patients were recruited. At baseline, clinical history, lung function measurements, peripheral blood samples and induced sputum were obtained. Using sputum samples, we performed gene expression analysis of TLR2, TLR3, TLR4, NOD1, NOD2, RIG‐I and MDA‐5 by quantitative reverse transcription‐polymerase chain reaction in addition to quantitative bacterial culture. COPD exacerbations were assessed based on Anthonisen's criteria using symptom diaries for the following 1‐year period. Results: During 1‐year follow‐up period, 13 patients experienced at least one exacerbation, but 18 patients did not. Those with exacerbations tended to be more severe COPD and showed larger neutrophil fraction in their induced sputum. Among PRRs, only TLR3 gene expression was increased in COPD patients with exacerbation compared with those without exacerbations. Multivariate logistic regression analysis including neutrophil fraction and TLR3 gene expression as predictor variables demonstrated that only an increase of neutrophil fraction, but not TLR3 gene expression, was a significant predictor for COPD exacerbation. Conclusion: TLR3 expression in inflammatory cells might affect the susceptibility to COPD exacerbation.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><country name="Japon"><region name="Région du Kansai"><name sortKey="Kinose, Daisuke" sort="Kinose, Daisuke" uniqKey="Kinose D" first="Daisuke" last="Kinose">Daisuke Kinose</name></region><name sortKey="Chin, Kazuo" sort="Chin, Kazuo" uniqKey="Chin K" first="Kazuo" last="Chin">Kazuo Chin</name><name sortKey="Hirai, Toyohiro" sort="Hirai, Toyohiro" uniqKey="Hirai T" first="Toyohiro" last="Hirai">Toyohiro Hirai</name><name sortKey="Hoshino, Yuma" sort="Hoshino, Yuma" uniqKey="Hoshino Y" first="Yuma" last="Hoshino">Yuma Hoshino</name><name sortKey="Kiyokawa, Hirofumi" sort="Kiyokawa, Hirofumi" uniqKey="Kiyokawa H" first="Hirofumi" last="Kiyokawa">Hirofumi Kiyokawa</name><name sortKey="Kudo, Megumi" sort="Kudo, Megumi" uniqKey="Kudo M" first="Megumi" last="Kudo">Megumi Kudo</name><name sortKey="Marumo, Satoshi" sort="Marumo, Satoshi" uniqKey="Marumo S" first="Satoshi" last="Marumo">Satoshi Marumo</name><name sortKey="Mishima, Michiaki" sort="Mishima, Michiaki" uniqKey="Mishima M" first="Michiaki" last="Mishima">Michiaki Mishima</name><name sortKey="Muro, Shigeo" sort="Muro, Shigeo" uniqKey="Muro S" first="Shigeo" last="Muro">Shigeo Muro</name><name sortKey="Ogawa, Emiko" sort="Ogawa, Emiko" uniqKey="Ogawa E" first="Emiko" last="Ogawa">Emiko Ogawa</name><name sortKey="Ogawa, Emiko" sort="Ogawa, Emiko" uniqKey="Ogawa E" first="Emiko" last="Ogawa">Emiko Ogawa</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001031 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001031 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:94F27A970F6FC3B47ED89F596AFC6157509AD2DA
|texte= Association of COPD exacerbation frequency with gene expression of pattern recognition receptors in inflammatory cells in induced sputum
}}
| This area was generated with Dilib version V0.6.33. |  |